New Step by Step Map For principle of hplc in pharma
The stationary phase is hydrophobic, Whilst the adaptable stage is often a polar liquid, including h2o-methanol or acetonitrile mixes. It is based about the hydrophobic collaboration rule, which states the much more nonpolar the compound, the more It will probably be retained.In Liquid Chromatography or HPLC, sample factors are divided based on the